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Isolated white adipocytes were stimulated for 4 h with or without 500 nM G49 in PBS-0.1% BSA

However, for concentrations of 3.1 M and 6.2 M, M4 completely blocked filamentation, probably killing the cells, as demonstrated by cell permeability test performed with Sytox Orange staining (Figure 5A)

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However, cell-laden hydrogels usually show low mechanical properties and cannot maintain their structural integrity by endoscopy delivery systems, which makes them unsuitable for arthroscopy treatment